• ADIAPURE™

ADIAPURE™

DNA extraction/purification kits for veterinary diagnostics

 

  • Easy-to-use
  • Complete solution
  • Validated processes in combination with AdiavetTM PCR kits
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ADIAPURE™ DNA extraction methods 

ADIAVETTM kits contain only amplification PCR reagents. In order to achieve very good performances on a wide range of samples, we recommend and validate many different DNA/RNA global extraction methods. It is very useful to stay flexible but without decreasing any performances (sensitivity, inhibition rate).

Today DNA and RNA extracts usable with ADIAVETTM kits can be prepared with :

Different technologies of purification : silica membrane, magnetic beads, ultra-filtration

Different automated system : centrifugation, vacuum, magnetic

For different throughputs : from individual analysis to 96 samples extraction per run

For some specific applications we propose our own extraction method with ADIAPURETM range in combination with ADIAVETTM PCR kits :

 

  • ADIAPURETM purification kit: for DNA extraction of Avian mycoplasmas and Ornithobacterium rhinotracheale from swabs
  • ADIAPURETM milk kit: for DNA extraction of Mycobacterium avium subsp. paratuberculosis from dairy products 
  • ADIAPURETM TLB buffer: single lysis buffer for RNA extraction of BVD virus from ear notches samples

 

Samples preparation steps (individual or pool) are developed and validated to be adapted to different epidemiological situations : diagnostic, individual analysis, screening with pool of samples or environmental samples.

ADIAPURE™ kits :

 

  • ADIAPURETM PURIFICATION KIT
  • ADIAPURETM MILK KIT
  • ADIAPURETM TLB BUFFER

Find out more details on the ADIAPURE™ DNA purification range on the technical details tab.

Why choose ADIAPURE™ for DNA purification in veterinary diagnostic?

PERFORMANCE : ADIAPURE™ kits are fully validated with ADIAVETTM Real Time PCR kits and improve their performances (level of inhibition, sensitivity)

 

EASY TO USE : ADIAPURETM protocols are simple and adapted to a routine buzzy diagnostic laboratory

 

HIGH THROUGHPUT : Direct lysis method or 96 wells-plate purification system allow to treat a lot of samples all together

DNA/RNA extraction step

In order to achieve very good performances on a wide range of samples, we recommend and validate many different DNA/RNA global extraction methods. It is very useful to stay flexible but without decreasing any performances (sensitivity, inhibition rate).

In this objective also, ADIAPURETM protocols have been developed for some specific veterinary applications where other common DNA extraction methods didn’t give expected results in term of performances and/or throughput.

Kits include all components for DNA extraction and/or purification.

Find out more details on equipment requested for using ADIAPURE™ DNA purification range on the option & accessories tab.

AVIAN DIAGNOSTIC

ADIAPURE™ PURIFICATION KIT

Pathogen

Samples

Lysis

Purification

Storage

 

Avian mycoplasmas and Ornithobacterium rhinotracheale

 

Swabs (tracheal or tissues). Pooling (up to 6 swabs)

 

Proteinase K

Filtration on 2 filters (96 wells) with a vacuum pump and a manifold

 

+2/8°C

 

RUMINANTS DIAGNOSTIC

ADIAPURE™ MILK KIT

 

Pathogen

Samples

Lysis

Purification

Storage

 

Mycobacterium avium subsp.paratuberculosis

 

Dairy products (milk and cheese)

Grinding (glass beads) and proteinase K

 

No purification step

 

+2/8°C

 

 

ADIAPURE TLB™ BUFFER

 

Pathogen

Samples

Lysis

Purification

Storage

BVD (Bovine Viral Diarrhea virus)

Ear notches (individual and pooling up to 20 samples)

ADIAPURETM TLB Buffer

 

No purification step

 

+2/8°C

 

For using ADIAPURETM purification kit

  • A manifold for 96-wells plates (eg : PALL). 

 

 

 

 

 

 

 

 

 

 

  • A vacuum pump adapted to manifold (0.9 mbar pressure minimum). (eg : KNF Series N 035.3 A_.18)

 

 

 

 

 

 

For using ADIAPURETM MILK kit

  • A magnet for 15mL/50ML tubes (eg : Qiagen, 36935)

 

  • A rotating homogenizer (eg : Stuart,SB3)

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